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TLR Function

Test Code: 403091P
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Clinical Utility

This test would be indicated in a patient with symptoms of immune deficiency when the routine immunological assessments have not been instructive.


Heparinized whole blood is stimulated with TLR ligands or left unstimualted (as background) and the cultured cells are incubated at 37°C. Following the stimulation phase, the plasma is recovered and analyzed for TNF-alpha by an Meso Scale Discovery (MSD®) Electrochemiluminescent Assay. This test has not been cleared or approved for diagnostic use by the U.S. Food and Drug Administration.

5-7 business days from receipt of specimen

Specimen Type Order Code CPT Code NY Approved Volume Assay Range Special Instructions
whole blood 403091P 86352 Yes 8 mL Varies by antigen/mitogen
  • 8 mL of whole blood. Collect and ship in original sodium heparin vacutainer. Blood should be mixed well to avoid clotting.
  • DO NOT USE lithium heparin, ACD tubes or EDTA anticoagulants.
  • Due to time constraints of the assay, samples need to be collected between the hours of 12 p.m. and 6 p.m.Central time, Monday through Thursday.
  • Ship samples via priority overnight courier at ambient temperature.
  • Temperature extremes must be avoided and the test must be performed within 24 hours of collection.

Specimens are approved for testing in New York only when indicated in the Specimen Information field above.

The CPT codes provided are based on Viracor Eurofins' interpretation of the American Medical Association's Current Procedural Terminology (CPT) codes and are provided for general informational purposes only. CPT coding is the sole responsibility of the billing party. Questions regarding coding should be addressed to your local Medicare carrier. Viracor Eurofins assumes no responsibility for billing errors due to reliance on the CPT codes illustrated in this material.

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