We use cookies to understand how you use our site and to improve your experience. Please refer to our Terms and Conditions and Privacy Policy for more information about the cookies we use and how we protect your privcy
Epstein-Barr Virus (EBV) Quantitative Real-time PCR
EBV is the etiologic agent of most post-transplant lymphoproliferative disorder (PTLD), which is an important cause of morbidity and mortality in both solid organ transplant recipients and HSCT patients. PTLD results from uncontrolled EBV-induced proliferation of B-cells in the immunocompromised setting. Quantitative EBV DNA PCR can be used to aid in the early diagnosis of PTLD, track the course of the disease, and monitor response to treatment.
Extraction of EBV viral DNA from specimen followed by amplification and detection using real-time, quantitative PCR. An internal control is added to ensure the extraction was performed correctly and the PCR reaction was not inhibited. Viracor Eurofins' assay design includes multiple targets to account for viral mutations, which significantly reduces the chance of false negative results. This test has not been cleared or approved for diagnostic use by the U.S. Food and Drug Administration.
The primers and probes used in this assay are specific for known EBV strains based on similarity search algorithms. Additionally, no cross reactivity was detected when tested against adenoviruses, BKV, CMV, HSV-1, HSV-2, HHV-6 variant A, HHV-6 variant B, HHV-7, HHV-8, JCV, parvovirus B19, SV-40, and VZV.
Same day (within 8 - 12 hours from receipt of specimen), Monday through Saturday.
Specimen Type | Order Code | CPT Code | NY Approved | Volume | Assay Range | Special Instructions |
---|---|---|---|---|---|---|
BAL | 4509 | 87799 | Yes | 2 mL (min. 0.5 mL) |
25 IU/mL to 1.69x108 IU/mL |
|
bone marrow | 4504 | 87799 | Yes | 2 mL (min. 0.5 mL) | 213 IU/mL to 1.69x108 IU/mL |
|
bronch wash | 4526 | 87799 | Yes | 2 mL (min. 0.5 mL) |
25 IU/mL to 1.69x108 IU/mL |
|
CSF | 4503 | 87799 | Yes | 2 mL (min. 0.5 mL) | 52 IU/mL to 1.69x108 IU/mL |
|
fecal | 4508 | 87798 | Yes | Size of pea, or 2 mL liquid stool | Detected/Not Detected |
|
plasma | 4501 | 87799 | Yes | 2 mL (min. 0.5 mL) |
49 IU/mL to 1.69x108 IU/mL |
|
serum | 4510 | 87799 | Yes | 2 mL (min. 0.5 mL) | 49 IU/mL to 1.69x108 IU/mL |
|
tissue [Quant] | 4505 | 87799 | Yes | 5 mg fresh tissue (approximately ½ of a pencil eraser size) | 7 IU/mg to 1.69x107 IU/mg |
|
tissue [Qual] | 4506 | 87798 | Yes | 5 mg fresh tissue (approximately ½ of a pencil eraser size) | Detected/Not Detected |
|
trach asp | 4519 | 87799 | Yes | 2 mL (min. 0.5 mL) |
25 IU/mL to 1.69x108 IU/mL |
|
trach wash | 4548 | 87799 | Yes | 2 mL (min. 0.5 mL) |
25 IU/mL to 1.69x108 IU/mL |
|
whole blood | 4516 | 87799 | Yes | 2 mL (min. 0.5 mL) | 64 IU/mL to 1.69x108 IU/mL |
|
Ship Monday through Friday. Friday shipments must be labeled for Saturday delivery. All specimens must be labeled with patient's name and collection date. A Viracor Eurofins test requisition form must accompany each specimen. Multiple tests can be run on one specimen. Ship specimens FedEx Priority Overnight® to: Viracor Eurofins, 1001 NW Technology Dr, Lee's Summit, MO 64086
Whole blood frozen, specimens beyond their acceptable length of time from collection as listed in the specimen handling, specimens received in trap containers or specimen types other than those listed.
Specimens are approved for testing in New York only when indicated in the Specimen Information field above.
The CPT codes provided are based on Viracor Eurofins' interpretation of the American Medical Association's Current Procedural Terminology (CPT) codes and are provided for informational purposes only. CPT coding is the sole responsibility of the billing party. Questions regarding coding should be addressed to your local Medicare carrier. Viracor Eurofins assumes no responsibility for billing errors due to reliance on the CPT codes illustrated in this material.
PCR tests are performed pursuant to a license agreement with Roche Molecular Systems, Inc.