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Adenovirus Quantitative Real-time PCR
Kim's Text
Adenovirus is an important cause of morbidity and mortality in the transplant setting, causing pneumonia, hemorrhagic cystitis, hepatitis, encephalitis, pancreatitis, enteritis, and disseminated disease with the mortality rate reaching 60% in some especially high risk situations such as pediatric hematopoietic stem cell transplantation. Since proper management is dependent upon early diagnosis, quantitative adenovirus DNA PCR is useful for detecting the virus, tracking the course of infection, and monitoring response to treatment.
Extraction of adenovirus DNA from specimen followed by amplification and detection of known adenovirus strains using real-time, quantitative PCR. An internal control is added to ensure the extraction was performed correctly and the PCR reaction was not inhibited. Viracor Eurofins' assay design includes multiple targets to account for viral mutations, which significantly reduces the chance of false negative results. This test has not been cleared or approved for diagnostic use by the U.S. Food and Drug Administration.
Detects all known serotypes in one assay. The primers and probes used in this assay are specific for known adenovirus strains based on similarity search algorithms. Additionally, no cross reactivity was detected when tested against BKV, CMV, EBV, HSV-1, HSV-2, HHV-6 variant A, HHV-6 variant B, HHV-7, HHV-8, JCV, Parvovirus B19, SV-40, and VZV.
Same day (within 8 - 12 hours from receipt of specimen), Monday through Saturday.
Specimen Type | Order Code | CPT Code | NY Approved | Volume | Assay Range | Special Instructions |
---|---|---|---|---|---|---|
BAL | 7509 | 87799 | Yes | 2 mL (min. 0.5 mL) |
38 copies/mL to 1x1010 copies/mL |
|
bone marrow | 7504 | 87799 | Yes | 2 mL (min. 0.2 mL) | 589 copies/mL to 1x1010 copies/mL |
|
bronch wash | 7526 | 87799 | Yes | 2 mL (min. 0.5 mL) | 38 copies/mL to 1x1010 copies/mL |
|
CSF | 7503 | 87799 | Yes | 2 mL (min. 0.5 mL) |
109 copies/mL to 1x1010 copies/mL |
|
fecal | 7508 | 87798 | Yes | Size of pea, or 2 mL liquid stool | Detected/Not Detected |
|
nasal asp | 7531 | 87799 | Yes | 2 mL (min. 0.5 mL) | 54 copies/mL to 1x1010 copies/mL |
|
nasal swab | 7530 | 87798 | Yes | 2 mL | Detected/Not Detected |
|
nasal wash | 7513 | 87799 | Yes | 2 mL (min. 0.5 mL) | 54 copies/mL to 1x1010 copies/mL |
|
NP aspirate | 7524 | 87799 | Yes | 2 mL (min. 0.5 mL) | 54 copies/mL to 1x1010 copies/mL |
|
NP swab | 7525 | 87798 | Yes | 2 mL | Detected/Not Detected |
|
NP wash | 7547 | 87799 | Yes | 2 mL (min. 0.5 mL) | 54 copies/mL to 1x1010 copies/mL |
|
pericardial fluid | 7512 | 87799 | Yes | 2 mL (min. 0.5 mL) | 157 copies/mL to 1x1010 copies/mL |
|
plasma | 7501 | 87799 | Yes | 2 mL (min. 0.5 mL) |
190 copies/mL to 1x1010 copies/mL |
|
pleural fluid | 7511 | 87799 | Yes | 2 mL (min. 0.5 mL) | 157 copies/mL to 1x1010 copies/mL |
|
serum | 7510 | 87799 | Yes | 2 mL (min. 0.5 mL) | 190 copies/mL to 1x1010 copies/mL |
|
throat gargle | 7523 | 87799 | Yes | 2 mL (min. 0.5 mL) |
54 copies/mL to 1x1010 copies/mL |
|
tissue [Qual] | 7506 | 87798 | Yes | 5 mg fresh tissue (approximately ½ of a pencil eraser size) | Detected/Not Detected |
|
tissue [Quant] | 7505 | 87799 | Yes | 5 mg fresh tissue (approximately ½ of a pencil eraser size) | 13 copies/mL to 1x1010 copies/mL |
|
trach asp | 7519 | 87799 | Yes | 2 mL (min. 0.5 mL) |
38 copies/mL to 1x1010 copies/mL |
|
trach wash | 7548 | 87799 | Yes | 2 mL (min. 0.5 mL) |
38 copies/mL to 1x1010 copies/mL |
|
urine | 7502 | 87799 | Yes | 2 mL (min. 0.5 mL) | 152 copies/mL to 1x1010 copies/mL |
|
Ship Monday through Friday. Friday shipments must be labeled for Saturday delivery. All specimens must be labeled with patient's name and collection date. A Viracor Eurofins test requisition form must accompany each specimen. Multiple tests can be run on one specimen. Ship specimens FedEx Priority Overnight® to: Viracor Eurofins, 1001 NW Technology Dr, Lee's Summit, MO 64086
Wood shafted swab, calcium alginate swab, specimens received in trap containers, specimens beyond their acceptable length of time from collection as listed in the specimen handling, or specimen types other than those listed.
Specimens are approved for testing in New York only when indicated in the Specimen Information field above.
The CPT codes provided are based on Viracor Eurofins' interpretation of the American Medical Association's Current Procedural Terminology (CPT) codes and are provided for informational purposes only. CPT coding is the sole responsibility of the billing party. Questions regarding coding should be addressed to your local Medicare carrier. Viracor Eurofins assumes no responsibility for billing errors due to reliance on the CPT codes illustrated in this material.
PCR tests are performed pursuant to a license agreement with Roche Molecular Systems, Inc.